线粒体OCR试剂是一种基于最新纳米技术研制的可主动靶向活细胞线粒体的成像试剂。该试剂生物相容性好，细胞毒性很低，可广泛应用于单细胞、细胞球Spheroids或者组织类器官等应用场景，实现细胞中氧气含量的测量。本公司所研制的线粒体OCR试剂具有优异的光稳定性，可通过荧光寿命测量技术实现细胞中氧浓度变化的实时跟踪，同时也能通过测量密封环境中细胞的氧气消耗情况，计算细胞的耗氧速率OCR，从而实现对细胞线粒体功能的实时评估。如中图所示，线粒体OCR试剂的Zeta电位为正，保证了试剂可以有效地逃离溶酶体，准确靶向到细胞线粒体上。同时试剂具有优异的光稳定性，在使用门控技术进行荧光寿命测量时，其可以稳定、可靠并且准确地测量细胞内氧浓度的动态变化，连续测量时间可长达数小时，如右图所示。 The mitochondrial oxygen consumption rate (OCR) reagent is an imaging reagent based on the latest nanotechnology that can actively target the mitochondria of living cells. The reagent has good biocompatibility and low cytotoxicity, and can be widely used in applications such as studying energy metabolism of single cells, cell spheroids or tissue organoids. The mitochondrial OCR reagent developed by our company has excellent photostability, and can achieve real-time tracking of oxygen concentration changes in living cells through fluorescence lifetime imaging technology. It can also calculate the oxygen consumption rate of living cells by measuring their oxygen consumption in a sealed environment, thus achieving real-time evaluation of the function of cellular mitochondria. As shown in the middle figure, the Zeta potential of the mitochondrial OCR reagent is positive, ensuring that the reagent can effectively escape from the lysosome and accurately target the mitochondria. At the same time, the reagent has excellent photostability, and when using gating technology for fluorescence lifetime measurement, it can stably, reliably, and accurately measure the dynamic changes in intracellular oxygen concentration, with continuous measurement time up to several hours, as shown in the right figure.

线粒体OCR试剂具有完全可逆的氧气响应行为，不仅可以通过荧光强度测量，而且可以通过测量荧光寿命准确定量线粒体中的氧浓度，该试剂完全兼容现有的时间分辨酶标仪设备，不需要额外的测量系统即可实现测量。试剂具有优异的响应灵敏度和响应速度，可在不到1s的时间内实现氧浓度的精准定量测量。 The mitochondrial OCR reagent has a completely reversible oxygen response behavior, which can be accurately quantified not only by measuring fluorescence intensity, but also by measuring fluorescence lifetime. This reagent is fully compatible with existing time-resolved microplate reader equipment for high throughput measurement. The reagent has excellent response sensitivity and response speed, and can achieve accurate quantitative measurement of oxygen concentration in less than a second.

线粒体OCR试剂不仅适用于高通量的96孔板进行细胞、药物和培养条件的筛选，也能进行显微成像，实现单/活细胞中氧浓度的测量，对类器官、组织切片也有很好的适用性。 The mitochondrial OCR reagent is not only suitable for high-throughput 96 well plate screening of cells, drugs, and culture conditions, but also for microscopic imaging, achieving measurement of oxygen concentration in single/live cells, and has good applicability to organoids and tissue sections.